Agrobacterium Mediated Transformation Optimizations for Sugarcane (Saccharum Officinarum L.) Cultivar SPF-234 with Direct Organogenesis

Sugarcane (Saccharum officinarum L.) is the most important food and energy crop worldwide. In present study, an efficient Agrobacterium mediated transformation regeneration system for sugarcane cultivar SPF-234 was established. tumefaciens strains EHA101and LBA4404 using vector pIG121 Hm, having GUS, HPTII NPTII genes were used. Polymerase chain reaction (PCR) histochemical assays confirmed GUS gene expression. A 620 bp fragment from positive plants amplified. The expressing putative transformants 35% of total formed under 30 minute immersion time 72 hr incubation period. co-cultivation media 60 µM acetosyringone produced 66% 58% EHA101. maximum average number directly shoot (59.5%) leaf explant in M6 1.00 mg/l 6-Benzylaminopurine (BAP) 2.5 Naphthaleneacetic acid (NAA). significant decrease (17%) observed when auxin (NAA) concentration increased to 4.0 mg/l. best response elongation SE4 equal (2.00 mg/l) both kinetin BAP. Increased concentrations significantly decreased subject cultivar. strain performed better genetic said cultivar.This quick less expensive direct could be exploited on commercial scale general, this elite particular.